Lighthouse Proteomics

Proteomics Services

Quantitative Profiling


Quantitative proteomics is a principal approach in understanding the complex dynamics within the proteome of a cell. Our LC-MS/MS methods can be used to explore the underlying molecular mechanisms behind biological processes, disease states, and drug treatment. We offer quantitative proteomic solutions to meet your diverse needs, utilizing LC-MS/MS technology over antibody-based methods (eg. ELISA). The accuracy and precision of our LC-MS/MS processes can be used for selective quantification of specific proteins (or PTM sites) through analysis of signature peptides in complex biological samples. These methods can identify hundreds to thousands of targeted peptides and proteins in a single LC-MS run, in a highly reproducible and quantitative manner.

Our Quantitative Proteome Profiling service is designed for the identification and quantification of proteins from either a cell culture or tissue samples. A denaturing protein lysate is generated from the sample and the resulting protein(s) are digested with trypsin (or other protease) to produce peptide fragments that are then analyzed with LC-MS/MS for identification and quantification to determine the relative protein abundance between control and treatment conditions.

How Can Lighthouse Proteomics Assist You With Your Research?

  • Characterize quantitative changes in proteins related to cell phenotype
  • Quantify protein changes related to treatment condition
  • Quantitatively monitor global protein levels upon compound treatment
  • Explore effect of compound treatment on global protein expression levels
  • Monitor protein expression through a series developmental stages
  • Identify key proteins for biomarker discovery
  • Identify regulated proteins to explore mechanism of action
  • Monitor proteins within a specific signaling pathway
  • Explore mechanism-of-action of drug
  • Identify PD biomarkers to monitor drug response or efficacy
You Provide: flash frozen tissue, cell culture slurry or cell pellet
We Provide: Protein quantification with high resolution LC-MS/MS technology
You Receive: A data package will be provided electronically in the form of an XLS result table(s) and a PowerPoint summary report. The data package is also accompanied with a consultation to review the results with you and address any questions you have about the analysis.
Our Quantitative Proteome-Wide PTM Profiling service is designed for the identification and quantification of protein PTMs (phosphorylation, acetylation, methylation, and ubiquitination) from either a cell culture or tissue samples. A denaturing protein lysate is generated from the sample and the resulting protein(s) are digested with trypsin (or other protease) to produce peptide fragments that are immunoaffinity enriched for the appropriate PTM containing peptides. The resulting peptides are analyzed by LC-MS/MS for identification and quantification to determine relative PTM peptide abundance between control and treatment conditions.

How Can Lighthouse Proteomics Assist You With Your Research?

  • Characterize quantitative changes in protein PTMs related to cell phenotype
  • Quantify protein PTM changes related to treatment condition
  • Quantitatively monitor global protein PTM levels upon compound treatment
  • Explore effect of compound treatment to phospho-signaling (or other PTMs)
  • Monitor protein-PTM status through a series developmental stages
  • Identify key protein-PTMs for biomarker discovery
  • Identify regulated protein-PTMs to explore mechanism of action
  • Monitor protein PTMs within specific signaling pathway
  • Examine extent of cross-talk among different PTMs
  • Explore mechanism-of-action of drug
  • Identify PD biomarkers to monitor drug response or efficacy
  • Monitor status of Histone PTMs in response to compound treatmenty
You Provide: flash frozen tissue, cell culture slurry or cell pellet
We Provide: Protein quantification with high resolution LC-MS/MS technology
You Receive: A data package will be provided electronically in the form of an XLS result table(s) and a PowerPoint summary report. The data package is also accompanied with a consultation to review the results with you and address any questions you have about the analysis.

PTMs include: Phosphorylation: S, T, Y; Acetylation; Succininylation; Methylation & Ubiquitination

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